Module for Paired-End Sequences
Paired end sequencing is a common technique used in NGS. It produces two reads from proximal locations of a target DNA or RNA molecule in both forward and reverse direction,which could be potentially utilized to enhance the alignment precise and coverage. Here, We present a method applying toRAPSearch2 that combine paired-end reads as one hit and evaluate the significance in the similarity search to improve sensitivity of alignment.
Based on the RAPSearch2 algorithm, we built a new module that could process the paired-end reads simultaneously. By using the paired end sequences aligned on the proximal locations on the same subject sequences, the method could increase the searching sensitivity by about 0.5%~0.6%, comparing to the similarity search by using each of the paired end sequences individually. |
FAQ
How can I cite this module?
How can I cite this module?
- Xiaoqian Zhang, and Haixu Tang. A New Module in RAPSearch2 for Fast Protein Similarity Search of Paired-end Sequences. IU CS Technical Report, Oct 2013